Molecularly imprinted polymers mips can be engineered for a variety of molecules, including as drugs such as paclitaxel. For diazinon extraction, high performance liquid chromatography analysis was demonstrated in this study. Molecularly imprinted polymers immobilized on 3d printed. Multitemplates molecularly imprinted polymer for the specific solid phase extraction of saponins from panax notoginseng herbal extract volume. Especially, mips have been applied to the extraction and separation of analytes as the selective adsorbent of solid phase extraction spe, which is known as molecularly imprinted polymer solid phase extraction mispe in recent years. Apr 02, 2008 the use of molecularly imprinted polymers for sampling and sample preparation. Automated online solid phase extraction coupled with sequential injectionhplcec system for the determination of sulfonamides in shrimp. Selective solidphase extraction of bisphenol a using molecularly imprinted polymers and its application to biological and environmental samples. Molecularly imprinted polymers mips can be applied to the cleanup and preconcentration of analytes as the selective adsorbent of solid phase extraction spe. Supelmip spe supelco molecularly imprinted polymer spe. Comparison of molecularly imprinted solidphase extraction with a mixedmode anion exchanger.
Solid phase extraction of dropropizine using molecularly. After optimization of molecularly imprinted solidphase extraction mispe method with 2 ml water plus 2 ml acetone as washing solvents and 5 ml of methanol and acetic acid 10. This technique is based on the system used by enzymes for substrate recognition, which is called the lock and key model. Mips, which have high selectivity and affinity for a predetermined molecule template, have been used as sorbents for spe to selectively isolate analytes from biological, pharmaceutical, and environmental samples. A novel method for extraction of galegine by molecularly.
Dualtemplate molecularly imprinted polymers for dispersive. Selective solid phase extraction of bisphenol a using molecularly imprinted polymers and its application to biological and environmental samples. Different kinds of deep eutectic solvents des based on choline chloride chcl and ionic liquids ils based on 1methylimidazole were used to modify fe 3 o 4 molecularly imprinted polymers fe 3 o 4 mips, and the resulting materials were applied for the rapid purification of alkaloid isomers theobromine and theophylline from green tea with magnetic solidphase extraction mspe. The hydrophilic molecularly imprinted dispersive solidphase extraction hmidspe coupled with liquid chromatography lc for the separation and the determination of azoxystrobin in cucumber was exploited. The nonmolecularly imprinted polymers nips were synthesized following the same procedure but without the template molecule raf. Pdf available in south african journal of chemistry. In recent years, a new type of spe has formed, molecularly imprinted polymer solid phase extraction mispe, and has been widely applied to the extraction of agrochemicals.
Molecularly imprinted polymers and their application in. Molecularly imprinted solid phase extraction of taxanes. Mips used for spe can be synthesized by three imprinting techniques and different methods of. Selective sorbents for solidphase extraction based on. Synthesis of dummytemplate molecularly imprinted polymer. Molecularly imprinted polymer solidphase extraction for. Molecularly imprinted polymers were synthesized using 1,4dihydroxy2naphthoic acid as the template and. Solid phase extraction spe based on molecularly imprinted polymers mips is a novel approach for sample preparation and preconcentration, gaining increased interest in the fields of environmental, clinical, and food analysis.
Alkali soil natural environmental science center, northeast forestry university, harbin 150040, p. Molecularly imprinted polymer nanoparticles for selective. Solid phase extraction is one of the major applications of molecularly imprinted polymers fields for cleanup of environmental and biological samples namely molecularly imprinted solidphase extraction. The smips were successfully employed as solidphase extraction. Molecularly imprinted polymers in solid phase extractions b. Molecularly imprinted polymer solid phase extraction followed by highperformance liquid chromatography as an efficient and sensitive technique for determination of meropenem in human plasma and urine. Groupselective molecularly imprinted polymer solidphase extraction for the simultaneous determination of six sulfonamides in aquaculture products. Mips for the flavonoid quercetin have been generated, using quercetin as a. Determination of citrinin using molecularly imprinted.
The liquidliquid extraction methods are not completely satisfactory because they are timeconsuming and require a tedious procedure. Molecularly imprinted polymers for solidphase extraction and. Solidphase synthesis of molecularly imprinted polymer. Pdf molecularly imprinted polymer solid phase extraction. By a mechanism of molecular recognition, mips are used as selective sorbents for solid phase extraction of target analytes from complex matrices. For full access to this pdf, sign in to an existing account, or purchase an annual subscription.
Development of solidphase extraction using molecularly. Caro e, marce rm, borrull f, cormack pag, sherrington dc. Pdf selective solidphase extraction of bisphenol a using. Magnetic solidphase extraction with fe3o4molecularly. Journal of chromatography b 2011, 879 1516, 10711076. The magnetic molecularly imprinted polymer mmip was synthesized as solid phase extraction spe sorbet to extract dbp from complex matrixes and as sensing element to improve the selectivity of. Pdf molecularly imprinted polymers mips as an innovative.
Its superior cleanup and selectivity provide fastersimpler sample prep methods, better ms compatibility reduced ion suppression allowing analysts to achieve. However, nowadays their use in solidphase extraction, socalled molecularly imprinted solidphase extraction mispe, is by far the most advanced technical application of mips. By a mechanism of molecular recognition, mips are used as selective sorbents for solidphase extraction of target analytes from complex matrices. A liquid chromatography based method to detect citrinin in corn was developed using molecularly imprinted solid phase extraction mispe sample cleanup. Development of solidphase extraction using molecularly imprinted polymer for the analysis of organophosphorus pesticides chlorpyrifos in aqueous solution binsalom, a. Shuying hou, songling yang, hongliang he, yan chen, jinhua wang and yang wang affiliation. Pimkwan chantarateepra, weena siangproh, shoji motomizu, orawon chailapakul. The molecular imprinting technique is a highly predeterminative recognition technology. Andersson li 2000b efficient sample preconcentration of bupivacaine from human plasma by solid phase extraction on molecularly imprinted polymers. Molecularly imprinted solid phase extraction as mentioned above, the noncovalent approach is the most widely used for the preparation of mips thanks to its versatility. In recent years, a new type of spe has formed, molecularly imprinted polymer solidphase extraction mispe, and has been widely applied to the extraction of agrochemicals. During optimization of the molecular imprinted solid phase extraction procedure for. Its application for separation and enrichment in solid phase extraction was investigated. Mips, which have high selectivity and affinity for a predetermined molecule template, have.
The technique of molecularly imprinted polymers to solid phase extraction mispe is. Dualtemplate molecularly imprinted polymers for dispersive solidphase extraction of fluoroquinolones in water samples coupled with high performance liquid chromatography wenhui lu, ac jie liu, bc jinhua li, c xiaoyan wang, c min lv, c rong cui b and lingxin chen cd. Molecularly imprinted solid phase extraction mispe. Comparison of molecularly imprinted solid phase extraction with a mixedmode anion exchanger. The first application combining mips with spe for advanced beverage analysis is reported. Molecularly imprinted polymers mips are tailormade antibody mimics obtained by a templating process at the molecular level 1,2,3. This paper reports a method using molecularly imprinted polymers that are grafted onto the surface of carboxylmodified multiwalled carbon nanotubes as the solidphase extraction adsorbents to detect rhodamine b in chili powder samples. Molecularly imprinted solidphase extraction as mentioned above, the noncovalent approach is the most widely used for the preparation of mips thanks to its versatility. Solid phase extraction with molecularly imprinted polymers mipspe is a promising technique which allows specific analytes to be selectively extracted from complex matrices. Surface molecularly imprinted polymers for solidphase. Synthesis of kaolinitemagnetic molecularly imprinted polymers for solid phase extraction of chlorpyrifos kunanon chattrairat and darinee phromyothin college of nanotechnology, king mongkuts institute of technology ladkrabang, bangkok 10520, thailand email.
Molecularly imprinted polymer as solid phase extraction. Molecularly imprinted polymer nanoparticles for selective solid phase extraction of fluvoxamine in human urine and plasma mojtaba soleimani. The use of mips as selective sorbent materials allows performing a customized sample treatment step prior to the final determination. Molecularly imprinted polymers, volume 23 1st edition. Application of molecularly imprinted polymers to solidphase extraction of compounds from environmental and biological samples. Development of an aflatoxin b1 specific molecularly imprinted. Abstract this paper presents the synthesis, optimization and application of a molecularly imprinted polymer mip sorbent for the selective extraction and preconcentration of the potent toxin, aflatoxin b1 afb1, from the child weaning food, tsabana manufactured in serowe, botswana. Molecularly imprinted polymer online solidphase extraction. Molecularly imprinted polymers mips are synthetic polymeric materials possessing specific cavities designed for a target molecule. Synthesis of kaolinitemagnetic molecularly imprinted. Supelmip spe is based on molecularly imprinted polymer mip technology.
Mar 10, 2009 molecularly imprinted polymers and their application in solid phase extraction martina lasakova department of analytical chemistry, faculty of chemical technology, university of pardubice, pardubice, czech republic. Advanced solid phase extraction using molecularly imprinted. A benefit of imprinted polymers is the possibility to prepare sorbents with selectivity predetermined for a particular substance, or group of structural analogues. Different kinds of deep eutectic solvents des based on choline chloride chcl and ionic liquids ils based on 1methylimidazole were used to modify fe3o4 molecularly imprinted polymers fe3o4mips, and the resulting materials were applied for the rapid purification of alkaloid isomers theobromine and theophylline from green tea with magnetic solid phase extraction mspe. Fast and costeffective molecularly imprinted polymer technique has applications in many fields of chemistry, biology and engineering, particularly as an affinity material for sensors, detection of chemical, antimicrobial, and dye, residues in food, adsorbents for solid phase extraction, binding assays, artificial antibodies, chromatographic. In this study, solid phase extraction using the imprinted polymer has been optimized with. For the mispe extraction system, 2 g of the synthesized mips and nip were placed in 6 ml cartridges. The application most close to a wider acceptance is probably that of solid phase extraction for cleanup of environmental and biological samples. It was found that, the imprinted polymer exhibited satisfactory performance in. Different molecularly imprinted polymers mips were prepared using noncovalent. Solid phase extraction with molecularly imprinted polymers mipspe is a. Nonimprinted polymers nips were also synthesized via the same procedure. The technique of molecularly imprinted polymers to solid phase extraction mispe is performance and high selectively, compared with traditional sorbents.
Molecularly imprinted polymers and their application in solid. Determination of a flame retardant hydrolysis product in human urine by spe and lcms. Molecular imprinting and solid phase extraction of flavonoid. A new selective material based on molecularly imprinted polymers mips was prepared and used as solidphase extraction spe sorbent for sample enrichment of organophosphorus pesticides opp residues prior to high performance liquid chromatography hplc. The influence of functional monomer, crosslinker, polymerization condition, porogen, and the. This paper describes the development of a molecularly imprinted polymerbased solid phase extraction mispe method coupled with enzymelinked immunosorbent assay elisa for determination of the pah benzoapyrene bap in vegetable oils. Multitemplates molecularly imprinted polymer for the. Molecularly imprinted polymers for solid phase extraction. Different kinds of deep eutectic solvents des based on choline chloride chcl and ionic liquids ils based on 1methylimidazole were used to modify fe 3 o 4 molecularly imprinted polymers fe 3 o 4 mips, and the resulting materials were applied for the rapid purification of alkaloid isomers theobromine and theophylline from green tea with magnetic solid phase extraction mspe. Hydrophilic molecularly imprinted microspheres hmims for azoxystrobin were synthesized through precipitation polymerization that hydroxypropyl methacrylate hpma as. Preparation and evaluation of coreshell magnetic molecularly.
Finally, the particles were washed with methanol to remove residual acetic acid, dried under vacuum at 70 c, and stored at room temperature. Development of molecularly imprinted polymers mips as a. Molecularly imprinted polymers mips as an innovative solidphase extraction sorbent for the analysis of antidepressants in environmental waters. Pdf selective solidphase extraction of bisphenol a. Each supelmip phase offers tailormade selectivity for the extraction of trace analytes in complex matrixes. Development of molecularly imprinted polymers mips as a solid phase extraction spe sorbent for the determination of ibuprofen in water y. Different kinds of deep eutectic solvents des based on choline chloride chcl and ionic liquids ils based on 1methylimidazole were used to modify fe3o4molecularly imprinted polymers fe3o4mips, and the resulting materials were applied for the rapid purification of alkaloid isomers theobromine and theophylline from green tea with magnetic solidphase extraction mspe. Molecularly imprinted solidphase extraction and liquid.
As a food safety regulatory measure, tsabana must be cleared of hazardous aflatoxins, especially afb1. Molecularly imprinted polymers mips can be applied to the cleanup and preconcentration of analytes as the selective adsorbent of solidphase extraction spe. Sep 14, 2011 caro e, marce rm, borrull f, cormack pag, sherrington dc. Development of an aflatoxin b1 specific molecularly. The active binding site of an enzyme has a shape specific to a substrate. Selectivity is introduced during mip synthesis in which a template molecule, designed to mimic the analyte, guides the formation of specific cavities or imprints that are sterically. Molecularly imprinted polymer solidphase extraction mispe in order to evaluate the selectivity of the mips, extractions were performed with standard catechin solution as tannin target analyte. In covalent imprinting, the print molecule the selective sorbents for solidphase extraction based on molecularly imprinted polymers kees ensing, mip technologies ab, lund, sweden and merska receptor screening methodologies. The author was also responsible for writing the major part of the paper.
Molecularly imprinted polymer mip against dropropizine was synthesized using dropropizine as template. Molecularly imprinted polymers for solidphase extraction. Especially, mips have been applied to the extraction and separation of analytes as the selective adsorbent of solidphase extraction spe, which is known as molecularly imprinted polymer solidphase extraction mispe in recent years. Andersson li 2000b efficient sample preconcentration of bupivacaine from human plasma by solidphase extraction on molecularly imprinted polymers. Determination of citrinin using molecularly imprinted solid. Molecularly imprinted polymers for selective solidphase. Molecularly imprinted solidphase extraction in the. The application of molecularly imprinted polymers to solid. Substrates with a complementary shape to the binding site.
Dualtemplate molecularly imprinted polymers for dispersive solid phase extraction of fluoroquinolones in water samples coupled with high performance liquid chromatography w. Molecularly imprinted solid phase extraction of taxanes from plant cell culture a major qualifying project submitted to the faculty of. Molecular imprinting and solid phase extraction of. Molecularly imprinted solid phase extraction of taxanes from. Molecularly imprinted polymers immobilized on 3d printed scaffolds as novel solid phase extraction sorbent for metergoline. Chimuka, applications of molecularly imprinted polymers for solidphase extraction of nonsteroidal antiinflammatory drugs and analgesics from environmental waters and biological samples, journal of pharmaceutical and biomedical analysis, vol. However, in parallel, such versatility is also the origin of some of the drawbacks attributed to mips. The imprinted polymers were found to have a highly specific recognition and binding capacity for. Methods for fluvoxamine by gcms, hplc with liquidliquid extraction and hplc with solidphase extraction 17, 18 were described in previous papers.
1534 52 60 716 454 469 1092 609 170 950 646 1227 1169 466 1304 1254 1283 991 480 1319 1382 915 1057 1515 1149 1534 1298 974 1265 724 987 44 333 270 840 153 1419 1363 121 61 1171 988